Accurate determination of genotypic variance of cell wall characteristics of a Populus trichocarpa pedigree using high-throughput pyrolysis-molecular beam mass spectrometry

Abstract Background Pyrolysis-molecular beam mass spectrometry (py-MBMS) analysis of a pedigree Populus trichocarpa was performed to study the phenotypic plasticity and heritability lignin content monomer composition. Instrumental microspatial environmental variability were observed in spectral features corrected reveal underlying genetic variance biomass Results Lignin-derived ions (including m/z 124, 154, 168, 194, 210 others) highly impacted by variation which demonstrates composition biomass. Broad-sense after correcting for instrumental determined be H 2 = 0.56 based on py-MBMS known derive from lignin. Heritability monomeric syringyl/guaiacyl ratio ( S / G ) 0.81. also high (up 0.79) derived other components including phenolics (e.g., salicylates) C5 sugars xylose). Lignin phenolic ion abundances primarily driven maternal effects, paternal effects either similar or stronger most heritable carbohydrate-derived ions. Conclusions We have shown that many biopolymer-derived show substantial response microenvironmental plantations. Nevertheless, broad-sense can quite spatial variation. This work outlines importance accounting data applications measurements genomic selection breeding poplar renewable fuels materials.